Regulation of Metalloprotease Gene Expression in Vibrio vulnificus by a Vibrio harveyi LuxR Homologue
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چکیده
منابع مشابه
LuxO controls luxR expression in Vibrio harveyi: evidence for a common regulatory mechanism in Vibrio.
Quorum-sensing control of luminescence in Vibrio harveyi, which involves an indirect autoinducer-mediated phosphorelay signal transduction system, contrasts with the prototypical quorum-sensing system of Vibrio fischeri, in which the autoinducer and the transcriptional activator LuxR directly activate lux operon expression. In V. harveyi, a regulator not homologous to V. fischeri LuxR and also ...
متن کاملIron-mediated regulation of metalloprotease VvpE production in Vibrio vulnificus.
In Vibrio vulnificus, the production of metalloprotease VvpE is controlled by Crp (cAMP-receptor protein) and SmcR (a quorum sensing regulator) at the transcription level, and by PilD-mediated type II general secretion system (TTGSS) at the extracellular secretion level. Iron is known to stimulate VvpE production but the related mechanisms remain unidentified. Iron stimulated vvpE transcription...
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Vibrio vulnificus is a marine bacterium and a human pathogen capable of causing wound infection and septicemia. We previously showed that the metalloprotease vEP secreted by V. vulnificus activates prothrombin in vitro. To further investigate the ability of vEP to activate other zymogens, we used a mutant form of procaspase-3 which lacks the native cleavage sites as a zymogen. The mutant zymoge...
متن کاملMetalloprotease is not essential for Vibrio vulnificus virulence in mice.
Previous work suggested that a metalloprotease, Vvp, may be a virulence factor of Vibrio vulnificus, which causes severe wound infection and septicemia in humans. To determine the role of Vvp in pathogenesis, we isolated an isogenic protease-deficient (PD) mutant of Vibrio vulnificus by in vivo allelic exchange. This PD mutant was as virulent as its parental strain in mice infected intraperiton...
متن کاملChemical modification of Vibrio vulnificus metalloprotease with activated polyethylene glycol.
Vibrio vulnificus, an opportunistic human pathogen causing septicemia, produces a metalloprotease which is suspected to be a virulence determinant, but which is labile in vivo due to inactivation by alpha-macroglobulin. To obtain a derivative which is stable in vivo, the metalloprotease was modified with activated monomethoxy polyethylene glycol. The modified protease retained full activity to ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2001
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.183.4.1369-1375.2001